Paramecium tetraurelia Assembly and Gene Annotation

About Paramecium tetraurelia

Paramecium tetraurelia is a unicellular ciliate, which is a model organism for the study of many biological processes. The Paramecium genome has diploid micronuclei and highly polyploid macronuclei (exact number unknown), is approximately 87Mbp in size, and has gone through at least three successive whole genome duplications.

Assembly

The genome of Paramecium tetraurelia strain d4-2 was sequenced by Genoscope in collaboration with the French National Research Organization and the French National DNA Sequencing Centre. The assembly was imported from the sequence submitted to the European Nucleotide Archive (assembly GCA_000165425). The 72-Mb draft assembly of the macronuclei is presented here.

Annotation

The protein-coding gene annotation of the Paramecium tetraurelia genome has been imported from that submitted to the European Nucleotide Archive (GCA_000165425).

Non-coding RNA genes have been annotated using tRNAScan-SE (Lowe, T.M. and Eddy, S.R. 1997), RFAM (Griffiths-Jones et al 2005), and RNAmmer (Lagesen K.,et al 2007); additional analysis tools have also been applied.

References

1. Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraurelia.
   Aury JM, Jaillon O, Duret L, Noel B, Jubin C, Porcel BM, Sgurens B, Daubin V, Anthouard V, Aiach N et al. 2006. Nature. 444:171-178.

Picture credit: Luis Fernàndez García