Hyaloperonospora arabidopsidis vs Phytophthora nicotianae P1569 LastZ results
Hyaloperonospora arabidopsidis (Hyaloperonospora arabidopsidis, HyaAraEmoy2_2.0) and Phytophthora nicotianae P1569 (Phytophthora nicotianae P1569, Phyt_para_P1569_V1) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl Protists release 111. Hyaloperonospora arabidopsidis was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Hyaloperonospora arabidopsidis | Phytophthora nicotianae P1569 |
|---|---|---|
| Chunk size | 10,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 10,100,000 | 10,100,000 |
| Masking options |
Statistics over 47,257 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Hyaloperonospora arabidopsidis |
Uncovered: 47,082,045 out of 78,897,814 |
Uncovered: 1,646,696 out of 13,171,834 |
| Phytophthora nicotianae P1569 |
Uncovered: 34,197,160 out of 55,229,644 |
Uncovered: 11,974,186 out of 27,601,672 |
Block size distribution
| Size range | All 47,257 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 342 |
1.6 kb |
|||
| 10 bp - 100 bp | 5,379 |
340.0 kb |
1,798 |
129.8 kb |
|
| 100 bp - 1 kb | 28,889 |
11.7 Mb |
14,676 |
6.1 Mb |
|
| 1 kb - 10 kb | 12,592 |
26.5 Mb |
8,841 |
24.9 Mb |
|
| 10 kb - 100 kb | 55 |
791.4 kb |
456 |
8.2 Mb |
|



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